澳门v泥斯

肖君华



1、个人简介

肖君华,男,19681013日出生, 民盟成员,东华大学生物科学与技术研究所 教授,博导

中国遗传学会理事,中国遗传学会产业委员会委员,中国遗传学会动物遗传专业委员会委员,上海市遗传学会常务理事。

研究方向:复杂性状相关基因的遗传学研究;野生小家鼠遗传资源的挖掘和利用,临床分子诊断技术的开发。

 

2、大学开始受教育经历

·          1985.9——1989.7   复旦大学遗传与遗传工程系,遗传与遗传工程系专业就学;获学士学位

·          1994.9——1997.7   复旦大学遗传所,获硕士学位   导师:柴建华教授

·          1997.9——2000.1   复旦大学遗传所, 获博士学位 导师:谈家桢院士 金力教授

3、研究工作经历

·          1989.9——1994.8   江苏省原子医学研究所,从事蛋白同位素标记试剂盒开发;

·          2000.3——2001.4   美国德洲大学休斯顿分校人类遗传中心博士后,从事多基因疾病及群体遗传学研究;

·          2001.4——2001.11   北京博宁生物工程科技有限公司执行副总裁,负责日常管理和科研开发的立项及管理;

·          2002.5——2007.7   华东理工大学 生物工程学院 生化所 副教授

·          2004.3――2007.7    东华大学 生物科学与技术研究所 医学分子遗传研究室 主任 副教授

·          2007.7――至今    东华大学 生物科学与技术研究所 医学分子遗传研究室 主任 教授

 

4、科研成果

作为课题负责人已完成的科研项目:

1.  基因表达、蛋白、组织芯片的研制与开发  分项目负责人    2002.102005.12  863基因功能与基因芯片重大专项      课题编号2002AA2Z2021

2. 中国人女性真性性早熟相关基因的研究      2004.12006.12 国家自然科学基金委员会青年科学基金项目  项目批准号30300198

3. 小鼠性发育启动调控基因的精细定位和克隆  20081-201012 国家自然科学基金委员会项目,项目批准号:30771191

4. 中国野生小家鼠群体遗传结构基本框架的研究 国家自然科学基金委员会基金项目 项目批准号:310710902011.01-2013.12

5. 小鼠近交系来源1号染色体替换系群体遗传特性研究  上海市创新行动计划 课题编号:121409004042012111-2015630

 

目前承担的项目:

利用野生小家鼠来源1号染色体替换系群体进行血脂代谢的系统遗传学研究  国家自然科学基金项目 项目批准号:31371257 2014.01-2017.12

 

发表论文:  *为通讯作者

1.Xu,Fuyi; Hu,Shixian; Chao,Tianzhu; Wang, Maochun; Li, Kai; Zhou, Yuxun; Xu, Hongyan; Xiao, Junhua*Sequence analysis of chromosome 1 revealed different selection patterns between Chinese wild mice and laboratory strains. Molecular genetics and genomics, DOI:10.1007/s00438-017-1335-z2017

2. Kan Yang, Bin Yu, Cheng Cheng, Tianlin Cheng, Bo Yuan, Kai Li, Junhua Xiao, Zilong Qiu & Yuxun Zhou*.Mir505–3p regulates axonal development via inhibiting the autophagy pathway by targetingAtg12. Autophagydoi.org/10.1080/15548627.2017.1353841, 2017

3.Maochun Wang, Li Tong , Sijia Wang , Kai Li, Junhua Xiao, Yuxun Zhou. A Multiplex Sensitive Quantification of MicroRNAs Based on Competitive PCR. Biotechnology and Bioprocess Engineering,22: 95-99. 2017

4.Ke Chen, Yuxun Zhou,  Kai Li,  Junhua Xiao*.Multiplex PCR with the blunt hairpin primers for next generation sequencing. Biotechnology and Bioprocess Engineering22:347-351. 2017

5.Fuyi Xu, Tianzhu Chao, Yiyin Zhang, Shixian Hu, Yuxun Zhou, Hongyan Xu, Junhua Xiao*, and Kai Li*. Chromosome 1 Sequence Analysis of  C57BL/6J-Chr1KM Mouse Strain. International Journal of Genomics. Volume 2017, Article ID 1712530, 9 pages https://doi.org/10.1155/2017/1712530

6.Xu F, Chao T, Liang Y, Li K, Hu S, Wang M, Zhou Y, Xu H, Xiao J*. Genome Sequencing of Chromosome 1 Substitution Lines Derived from Chinese Wild Mice Revealed a Unique Resource for Genetic Studies of Complex Traits. G3 (Bethesda). doi:10.1534/g3.116.0339022016

7. Ke ChenYu-xun ZhouKai LiLi-xin QiQi-fei ZhangMao-chun WangJun-hua Xiao*. A novel three-round multiplex PCR for SNP genotyping with next generation sequencing. Anal Bioanal Chem,408:43714377, 2016

8. Li TongHuihui Xue Li Xiong Junhua Xiao Yuxun Zhou*. Improved RT-PCR Assay to Quantitate the Pri-, Pre-, and Mature microRNAs with Higher Efficiency and Accuracy. Mol Biotechnol, 57:939–946, 2015

9. Yuxun Zhou, Yinming Liang, Kai Li, Xiong Bai , Guoqiang Chen, Zhenghong Xing and Junhua Xiao. The phenotypic distribution of quantitative traits in a wild mouse F1 population.  Mamm Genome, 23:232–240, 2012

10. Yuxun Zhou, Qinghua Guan, Kai Li, Lingyun Tao, Jianhua Hu, and Junhua Xiao*. Dissection of the maternal effects on puberty onset by embryo transplantation in mouse. J Endocrinolog Invest. 35:676-680, 2012.

11.Jia Li, Lihui Lin, JuanWang, Xia Peng, Yanan Liu, Junhua Xiao, Yuxun Zhou*, Li Li. Quantitative analysis of multiple genes’ expressions based on a novel competitive RT-PCR assay. Anal Bioanal Chem. DOI 10.1007/s00216-012-6518-1, 2012

12. Xianhuan Jiang, Jun Gao, Liju Ni, Jianhua Hu, Kai Li, Fengping Sun, Jianyun Xie, Xiong Bo, Chen Gao, Junhua Xiao, Yuxun Zhou*. The complete mitochondrial genome of Microtus fortis calamorum (Arvicolinae, Rodentia) and its phylogenetic analysis. Gene. 498: 288-295, 2012

13.Zhourui Tang, Kai Li, Yuxun Zhou*, Zhenxian Xiao, Junhua Xiao, Rui Huang, Guohao Gu. Comparative quantification of human intestinal bacteria based on cPCR and LDR/LCR  World J Gastroenterology. 18(3): 268-274, 2012

14. 陈烨,李凯,周宇荀*,肖君华小鼠基因转录表达分析中内参基因的优选。中国实验动物学报193):197-202, 2011

15.Ying Zhao, Ting Chen, Yuxun Zhou, Kai Li , Junhua Xiao*, An association study between the genetic polymorphisms within GnRHI, LHβand FSHβgenes and central precocious puberty in Chinese girls. Neuroscience Letters486 : 188–192, 2010

16.Junhua Xiao*, Yinming Liang, Kai Li, Yuxuan Zhou, Wenqian Cai, Yumei Zhou, Ying Zhao, Zhenghong Xing, Guoqiang Chen and Li Jin. A novel strategy for genetic dissection of complex traits: the population of specific chromosome substitution strains from laboratory and wild mice.Mamm Genome Vol 21, 370-376, 2010

17.Liya Zhao, Kai Li, Shimin Bao, Yuxun Zhou, Yinming Liang, Guoji Zhao, Ye Chen and Junhua Xiao.A 1-bp deletion in the γC-crystallin leads to dominant cataracts in mice. Mamm Genome  Vol 21, 361-369, 2010

18.Yong-Zhong Wang, Jun-Hua Xiao, Li-Hua Ruan, Hui-Yin Zhang, Min Chen, Xiang-Ke Pu ,Hong-Yu Shen, Guo-Xiang Wu.  Detection of the rtA181V/T and rtN236T mutations associated with resistance to adefovir dipivoxil using a ligase detection reaction assay. Clinica Chimica Acta 408 : 70–74,2009

19. 王勤熙, 李凯*,周宇荀, 肖君华. 基于荧光通用引物的多重定量RT-PCR基因表达谱分析平台的构建、优化及其应用.遗传, 31 (5): 552―561,2009

20. Jianhui WangMinghui YuKai Li Junhua XiaoYuxun Zhou*.Improved PCR-BSP Assay for Multiplex Methylation Pattern Analysis in Minimal Amount of DNA. Mol Biotechnol42(3): 333-340,2009

21. Li Kai, Bei Chen, Yuxun Zhou, Rui Huang, Yinming Liang, Qinxi Wang, Zhenxian Xiao, Junhua Xiao*. Multiplex quantification of 16S rDNA of predominant bacteria group within human fecal samples by polymerase chain reaction-ligase detection reaction (PCR-LDR). Journal of Microbiological Methods, 76: 289-294,2009

22. Wangsheng Zhu, Zhongpeng Fan, Chao Zhang, Zhengxia Guo, Ying Zhao, Yuxun Zhou,Kai Li, Zhenghong Xing, Guoqiang Chen, Yinming Liang, Li Jin, Junhua Xiao*A Dominant X-Linked QTL Regulating Pubertal Timing in Mice Found by Whole Genome Scanning and Modified Interval-Specific Congenic Strain Analysis  PLoS ONE,83: e3021,2008

23. MingXing He Kai Li , JunHua Xiao, YuXun ZhouRapid bio-barcode assay for multiplex DNA detection based on capillary DNA AnalyzerJournal of Virological Methods 151 :126–131,2008

24. 范忠鹏, 朱旺升,张超, 周宇荀,李凯,梁银明, 邢正宏, 陈国强,柏熊, 肖君华*荧光-构象敏感凝胶电泳技术筛查上海市郊区野生小家鼠线粒体编码区SNP位点.遗传,30(4):475-482,2008

25.X. Luan, H. Yu, X. Wei, Y. Zhou, W. Wang, P. Li, X. Gan, D. Wei and J. Xiao*, GPR54 Polymorphisms in Chinese Girls with Central Precocious Puberty Neuroendocrinology 86:77–83,2007

26. Xiaohui Luan, Yuxun Zhou,WeiWang, Hong Yu, Pin Li, Xiaohong Gan, DongzhiWei and Junhua Xiao*Association study of the polymorphisms in the KISS1 gene with central precocious puberty in Chinese girls European Journal of Endocrinology 157 113–118,2007

27.Yuxun Zhou, Wangsheng Zhu, Zhengxia Guo, Ying Zhao, Zijun SongJunhua Xiao* Effects of Maternal Nuclear Genome on the Timing of Puberty of offspring in MiceJournal of Endocrinology 193405-412,2007

28. 郭正霞,周宇荀,胡亚慧,肖君华*哺乳期营养状况对小鼠性发育启动时间的影响研究华东理工大学学报Vol.33(2):186-189, 2007

29.Zhen-Xian Xiao, Hui-Min Cao, Xiao-Hui Luan, Jian-Long Zhao, Dong-Zhi Wei, Jun-Hua Xiao*Effects of Additives on Efficiency and Specificity of Ligase Detection Reaction  Molecular Biotechnology  February  Vol 352):129-134, 2007

30.Yichuan Xiao, Yuxun Zhou, Jianhui Wang, Gang Wang, Minghui Yu, Jian Jin, Junhua Xiao*, Selection and identification of GnRH promoter binding peptide by phage display-CEMSA  Journal of Molecular Recognition. Vol.20(1), Pages 51-57, 2007

31. Junhua Xiao, Xiujuan Xin, Xiaohui Luan, Dongzhi Wei1, Shengli Yang.A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing. Genet. Mol. Biol, vol.29, no.3, p.562-565, 2006

32.张世扬,肖振贤,赵建龙,肖君华*,曹慧敏基于连接酶检测反应的并行分型系统检测AGT M235TACE I/D基因多态性,华东理工大学学报Vol.32(9):1050-1054, 2006 

33.Xin X, Xiao J*, Luan X, Zhou Y, Lu D, Wei D, Yang S. Association study of six activity SNPS in adrenal steroid hormone metabolism and IBM related genes with precocious puberty in Chinese girls. Neuro Endocrinol Lett  Apr 25;27(1-2):219-224,2006

34.Jun-hua Shi, Yuxun Zhou, Ming-lei He, Jian Jin,Yi-chuan Xiao, Jun-hua Xiao*, and Dong-zhi Wei. Synthesis of a novel 18b-glycyrrhetinic acid derivative. Chemistry of Natural Compounds, Vol. 42(3):325327,2006(ISSN: 0009-3130)

35.Zhenxian Xiao, Junhua Xiao, Yuexing Jiang, Shiyang Zhang, Minghui Yu, Jianlong Zhao, Dongzhi Wei, Huimin Cao. A Novel Method Based on Ligase Detection Reaction for Low Abundant YIDD Mutants Detection in Hepatitis B Virus. Hepatology Research 34 :150–155,2006. (ISSN: 1386-6346)

36. Xiujuan Xin, Xiaohui Luan, Junhua Xiao*, Dongzhi Wei, Jianhui Wang, Daru Lu, Shengli Yang,  Association study of four activity SNPs of CYP3A4 with the precocious puberty in Chinese girls.Neuroscience letter Vol.381(3):284-288,2005

37.吴弘;肖君华; 蔡刚明; 胡芳;徐红岩; 卢大儒; 薛京伦;金力;高血压病患者β2-肾上腺素能受体基因5'-调控区单核苷酸多态性研究  中华医学杂志. 81(1).23-26,2001

38.柯越海;宿兵; 肖君华; 陈华;黄薇; 陈竺;褚嘉佑; 谈家祯;金力; 卢大儒 Y染色体单倍型在中国汉族人群中的多态性分布与中国人群的起源及迁移中国科学·C2000V30N6 614-620

39.Xiao J, Hu F, Xu H, Jiang Y, Luo J, Zhang W, Qiu X, Xue J, Tan CC, Jin L. Provincial distribution of three HIV-1 resistant polymorphisms (CCR5-Delta32, CCR2-64I, and SDF1-3’A). Science in China C 2000.43:16-20.

40.Su B, Xiao C, Deka R, Seielstad MT, Kangwanpong D, Xiao J, Lu D, Underhill P, Cavalli-Sforza LL,  Chakraborty R, Jin L  Y chromosome haplotypes reveal prehistorical migrations to the Himalayas. Human Genetics. 2000.107:582-590

41.Su B, Deka R, Xiao C, Xiao J, Underhill P, Cavalli-Sforza L, Charkaborty R, Jin L. The origin and genetic affinity of Sino-Tibetan speaking populations. American Journal of Human Genetics 1999.65: 2260.

42.Jin L, Su B, Xiao J, Underhill PA. Ancient human migrations in East Asia as shown by Y haplotypes. Human Evolution, 1999. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

43. Sun G, Su B, Xiao J, Hu F, Lu D, Chakraborty R, Deka R, Jin L. Estimation of relative hazard for AIDS based on distributions of three HIV-1 resistant polymorphisms (SDF1-3'A, CCR2-641, CCR5-32) in global populations. American Journal of Human Genetics 1999.65:456.

44. Su B, Xiao J, Underhill P, Deka R, Zhang W, Akey J, Huang W, Shen D, Lu D, Luo J, Chu J, Tan J, Shen P, Davis R, Cavalli-Sforza L, Chakraborty R, Xiong M, Du R, Oefner P, Chen Z, Jin L Y-Chromosome evidence for a northward migration of modern humans into Eastern Asia during the last Ice Age. Am J Hum Genet 1999 Dec;65(6):1718-24

45.Su B, Chakraborty R, Jin L, Xiao J, Lu D. An HIV-resistant allele is exceptionally frequent in New Guinean highlanders. The Journal of the American Medical Association 1998 Dec 2;280(21):1830 

  

 EMAIL:xiaojunhua@dhu.edu.cn 


澳门v泥斯